Construction of a Genetic Engineering Strain That Can Produce Extracellular Polyssacharidesin Higher Temperature for MEOR

Journal of Petrochemical Universities ›› 2007, Vol. 20 ›› Issue (4): 25-27.

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Construction of a Genetic Engineering Strain That Can Produce Extracellular Polyssacharidesin Higher Temperature for MEOR

YI Wen-jing, ZHANG Zhong-zhi** , SUN Shan-shan, WEI Xiao-fang, LUO Yi-jing

Faculty of Chemical Science and Engineering，China Petroleum University, Beijing 102249,P.R.China

Received:2006-09-22 Published:2007-12-20 Online:2017-06-28

提高原油采收率的耐热产多糖基因工程菌构建

伊文婧, 张忠智** , 孙珊珊, 魏小芳, 罗一菁

中国石油大学化工学院,北京102249

作者简介:伊文婧(1982-),女,山东临沂市,硕士。
基金资助:
2006年度中石油股份公司科技风险创新基金项目。

Abstract

Abstract:

A strain screened from the waste water of an oilfield can produce insoluble extracellular polysaccharide used in selective plugging which is instrumental in microbially enhanced oil recovery. However this strain is sensitive to high temperature and produces little extracellular polyssacharides above 40 ℃. Therefore pET-ecoRRF, pET-tteRRF and corresponding control plasmids were transformed into the strain. The new strain can grow in 45 ℃, and western-blot indicates that the RRF genes have been expressed noticeably in the transgene strains. It has been showed that the transgene strains are able to bear higher temperature, and the sustainable temperature is higher five to eight degrees than the original strain. Because Enterobacter sp. is related to Escherichia coli, the expression of RRF is better than the gene of Thermus thermophilus in the strain of JD. At last it is successful to get a genetic engineering microbe which can endure the temperature as high as 45 ℃ and produce extracellular polysaccharides.

Key words: Microbially enhanced oil recovery(MEOR) , , Insoluble polysaccharide , , Genetic engineering microbe , , Thermophile

摘要： 为了提高用于微生物采油的掺水不溶性多糖的JD菌的耐温性能,将嗜热质粒pET-ecoRRF和pET
-tteRRF转化至菌株JD中,用卡纳霉素筛选转化子。在耐热性初步鉴定中,所构建的菌株可在45 ℃下生长;再进
行Western-blot检测确认,结果表明,RRF基因有高量的表达。RRF基因转化到JD菌中有较好的表达,提高了目
标菌株的耐热性能,耐热范围提高了5~8 ℃。由于Enterobactersp.和Escherichiacoli 的亲缘关系较近,所以将
Escherichiacoli 中的RRF基因在JD菌中表达效果比Thermusthermophilus 中的基因表达效果要好。最终将野生
JD菌改造成了一株能够在45℃耐热、产多糖的基因工程菌。

关键词: 微生物提高原油采收率 , , 多糖 , , 基因工程菌 , , 嗜热

YI Wen-jing, ZHANG Zhong-zhi*,SUN Shan-shan, WEI Xiao-fang, LUO Yi-jing.

Construction of a Genetic Engineering Strain That Can Produce Extracellular Polyssacharidesin Higher Temperature for MEOR

[J]. Journal of Petrochemical Universities, 2007, 20(4): 25-27.

伊文婧, 张忠智*,孙珊珊, 魏小芳, 罗一菁. 提高原油采收率的耐热产多糖基因工程菌构建[J]. 石油化工高等学校学报, 2007, 20(4): 25-27.

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Construction of a Genetic Engineering Strain That Can Produce Extracellular Polyssacharidesin Higher Temperature for MEOR

提高原油采收率的耐热产多糖基因工程菌构建

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