关键词: 低温 , 烷烃降解菌 , 摄取模式 , 细胞表面亲油性

Abstract: Carbon energy utilization, cell surface hydrophobicity and broth emulsion capability methods were adopted to study uptake modes on three types of low temperature alkanes-degrading microbes, A(Yarrowia sp.), H(Rhodococcus sp.), LD2(Acinetobacter sp.) to assimilate petroleum hydrocarbon. The results show that microbe A and H exhibited good capability to utilize hydrophilic carbon energy, broth OD600 values are 1.85～2.12 and 1.41～1.54 respectively at stale period, and microbes also utilized hydrophobic carbon energy well, broth OD600 values are 0.68～0.80 and 0.63～0.68. Microbe cell surface hydrophobicity reached maximum values at logarithmic growth phase, MATH values are 0.69 and 0.61. Microbes can produce surfactant to make oil layer emulsion and presented large amount of microdroplets suspended in water phase, the maximum height ratio of emulsion layer to total layers are 0.94 and 0.96, so uptake mode on microbe A and H belong to interaction between cell and petroleum microdroplets. Microbe LD2 has poor capability to utilize hydrophilic carbon energy, broth OD600 values are only 0.48～0.58 at stable period, but LD2 [KG*4]utilized [KG*4]hydrophobic [KG*4]carbon [KG*4]energy [KG*4]well, [KG*4]broth OD600 values are 0.78～0.82. Microbe had a higher cell surface hydrophobicity at all growth periods, MATH values are 0.72~0.85, oil layer demonstrated none emulsion, therefore, uptake mode on microbe LD2 belongs to direct contact between cell and petroleum large particles.

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