Abstract:

The biosurfactant produced by Pseudomonas aeruginosa strain KO5-2-9 could reduce surface tension to 26.6 mN/m and emulsified diesel up to 72.6% with the critical micelle concentration of 85.82 mg/L. Fourier transform infrared spectrum of extracted biosurfactant indicated the key component was glycolipid. The surface tension (ST) and emulsification index (E₂₄) were taken as evaluation indexes to study on stability of surfactants. The results showed that compared with chemical surfactants, the biosurfactant had high surface activity and emulsifying ability in high temperature, high salinity and high alkalinity. The results of physical simulation experiment indicated that the biosurfactant could enhance oil recovery over 8% and would have a good prospect in microbial enhanced oil recovery.

Key words:

iosurfactant, Glycolipid, Microbial enhanced oil recovery, Physical simulation

摘要： 铜绿假单胞菌(Pseudomonasaeruginosa)KO5-2-9所产表面活性剂能将水的表面张力降至26.6
mN/m,对柴油的乳化指数为72.6%,临界胶束浓度(CMC)为85.82mg/L。红外光谱分析显示该表面活性剂为一
种糖脂类生物表面活性剂。以表面张力(ST)和乳化指数(E₂₄)为评价指标,对比生物表面活性剂和化学表面活性剂
对温度、矿化度和酸碱度的稳定性,结果表明生物表面活性剂在高温、高矿化度以及高碱度的极端环境下仍具有较
高的表面活性和乳化能力,较化学表面活性剂更具优势。生物表面活性剂物理模拟驱油实验提高采收率8%以上,
在微生物采油中具有广阔的应用前景和重要的利用价值。

关键词: 生物表面活性剂, 糖脂类, 微生物采油, 物理模拟