Preparation of GO/CS Blend Membrane for Adsorption of Bilirubin

doi:10.12422/j.issn.1672-6952.2025.03.002

Abstract

Abstract:

In order to solve the problems of poor blood compatibility and low adsorption capacity of chitosan (CS) when using graphene oxide (GO) alone, a graphene oxide/chitosan blend membrane (GO/CS membrane) was prepared. Microscopic morphology and composition analysis were conducted using SEM and FTIR, and bilirubin adsorption experiments were carried out on GO/CS membranes. The results showed that when the mass fraction of GO in the casting solution was 3%, the adsorption capacity of GO/CS membrane for bilirubin was optimal. The absorption intensity of C=O belonging to the amide group increases near 1 650 cm^-1, while the stretching vibration peak of -NH₂ at 3 353 cm^-1 and the bending vibration peak of N-H at 1 570 cm^-1 weaken simultaneously. Additionally, there is no stretching vibration peak of carboxyl group C=O near 718 cm^-1, indicating that an amide reaction has occurred between GO and CS molecules, and the GO/CS membrane has been successfully prepared. Under the conditions of a reaction temperature of 37 ℃ and an adsorption time of 120 min, the GO/CS membrane exhibits the best bilirubin adsorption effect, with a saturated adsorption capacity of 77.8 mg/g. Increasing the mass concentration of bilirubin and lowering the pH under alkaline conditions are both beneficial for adsorption. Increasing the ionic strength of the solution or the mass concentration of bovine serum albumin is not conducive to the adsorption of bilirubin.

Key words: Membranes, Chitosan, Graphene oxide, Adsorption, Bilirubin

摘要：

为了解决单独使用氧化石墨烯（GO）时血液相容性差和壳聚糖（CS）吸附能力低的问题，制备了一种氧化石墨烯/壳聚糖共混膜（GO/CS膜）；利用SEM、FTIR进行了微观形态及组成分析，并进行了GO/CS膜的胆红素吸附实验。结果表明，当铸膜液中GO质量分数为3%时，GO/CS膜对胆红素的吸附能力最优；1 650 cm^-1附近归属于酰胺基团的C=O吸收强度增大，3 353 cm^-1处的-NH₂的伸缩振动峰和1 570 cm^-1处的N-H的弯曲振动峰同时减弱，并且在1 718 cm^-1附近没有出现羧基C=O的伸缩振动峰，说明GO和CS分子之间发生了酰胺反应，GO/CS膜制备成功；在反应温度为37 ℃、吸附时间为120 min的条件下，GO/CS膜的胆红素吸附效果最优，其饱和吸附量为77.8 mg/g；提高胆红素质量浓度，在碱性条件下降低pH，均有利于吸附；增大溶液的离子强度或牛血清白蛋白质量浓度，不利于胆红素的吸附。

关键词: 共混膜, 壳聚糖, 氧化石墨烯, 吸附, 胆红素

CLC Number:

TQ028.8

Xinyu WANG, Pengfei KONG, Zhongyan WANG, Feixue LIANG, Jia JU. Preparation of GO/CS Blend Membrane for Adsorption of Bilirubin[J]. Journal of Liaoning Petrochemical University, 2025, 45(3): 11-17.

王馨雨, 孔鹏飞, 王忠岩, 梁飞雪, 鞠佳. GO/CS膜的制备及其吸附胆红素的研究[J]. 辽宁石油化工大学学报, 2025, 45(3): 11-17.

Figures/Tables 8

Fig.1 Effect of GO mass fraction on bilirubin adsorption by GO/CS membranes

Fig.2 SEM images of GO, GO/CS membrane surfaces and sections

Fig.3 Infrared spectra of GO, CS and GO/CS membranes

Fig.4 Effects of adsorption time and temperature on bilirubin adsorption by GO/CS membranes

Fig.5 Effect of mass concentration of bilirubin on bilirubin adsorption by GO/CS membranes

Fig.6 Effects of pH on bilirubin adsorption by GO/CS membranes

Fig.7 Effects of NaCl concentration on bilirubin adsorption by GO/CS membranes

Fig.8 Effects of bovine serum albumin mass concentration on bilirubin adsorption by GO/CS membranes

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