Abstract: A glycolipids type biosurfactant, as identified by FTIR was obtained from Enterobacter cloacae, a petroleum-degrading bacterium. The optimum productivity of the biosurfactant was obtained. An optimal yield of biosurfactant was achieved at a n(C)/n(N) ratio of 8.9 using glucose as carbon source and ammonium chloride as nitrogen source when the initial pH value of the culture media is 7.2. It is shown that the presence of the biosurfactant greatly enhanced water-solubility of phenanthrene and the removal of phenanthrene from kaolinite soil. These would thus improve the bioavailability of phenanthrene and intensify the bioremediation of petroleum contaminated soil.

Key words: Enterobacter cloacae, Biosurfactant, Glycolipids, Phenanthrene

摘要： 对阴沟肠杆菌（Enterobacter cloacae）分泌表面活性剂的行为进行了初步研究，结果表明E.cloacae能以葡萄糖等水溶性碳源产生糖脂类生物表面活性剂，以葡萄糖为碳源、氯化铵为氮源时其最佳培养基组成的质量分数分别是3%、0.1%，碳氮比为8.9，初始pH值为7.2。该生物表面活性剂对菲有较强的增溶效果，并可强化高岭土所吸附的菲的脱附，可望促进石油烃污染土壤的生物修复过程。

关键词: 阴沟肠杆菌, 生物表面活性剂, 糖脂, 菲