Abstract: 627 strains of Actinomyces were separated from the eastern part mountainous area in Fushun Liaoning by platescreening, and antagonism in agar plate, fermented liquid and its antagonistic effect were all investigated. The results indicate that YYHS-2 showed strongly antagonistic effect against Staphylococcus aureus, Bacillius subtilis, Escherichia coli and Streptococcus mutans. The morphological, culture, physiological and biochemical characteristics of YYHS-2 all met the requirements of the definition of Streptomyces in Yan Xun-chu's taxonomy system，it's obvious that YYHS-2 belongs to Streptomyces.

Key words: Soil actinomyces, Actinomyces platescreening, Screen for antagonistic effect, Streptomyces

摘要： 采用平板分离法从抚顺东部山区不同植被类型土壤中分离出627株放线菌，对其进行皿内琼脂平板、发酵液及拮抗菌的拮抗性筛选。结果表明，YYHS-2等菌株对金黄色葡萄球菌(Staphylococcus aureus)、枯草芽孢杆菌(Bacillius subtilis)、大肠杆菌(Escherichia coli)、变形链球菌(Streptococcus mutans)等4种供试病原真菌具有强烈的拮抗作用。根据YYHS-2菌株的形态特征、培养特征、生理生化特征及细胞壁化学成分分析，均符合阎逊初放线菌分类系统中关于链霉菌的定义，可确定YYHS-2菌株归属于链霉菌属(Streptomyces)。

关键词: 土壤放线菌, 拮抗性筛选, 放线菌分类鉴定, 链霉菌属