Abstract: Temperatureresponsive culture substrates containing poly(Nisopropylacrylamide) (PNIPAAm) have been developed as an effective substitute for enzymatic treatment to recover adherent cells, which can control cell attachment and detachment by changing temperature. However, not all PNIPAAmcontaining surfaces can be applicable to cell culture and recovery. That means adherent cells show accommodative selectivity for thermoresponsive substrates. In this study, different cells including HeLa cells, HEK293 cells, bone marrow mesenchymal stem cells (BMMSCs) and adiposederived stem cells (ADSCs) were cultured on the temperatureresponsive PNIPAAm copolymer films with different surface wettability, topography and film thickness. The experimental results demonstrate that various cells had their special thermoresponsive substrates, and the optimal copolymer films were selected for HeLa cells, HEK293 cells, BMMSCs and ADSCs respectively.

Key words: Poly (Nisopropylacrylamide) (PNIPAAm), Bone marrow mesenchymal stem cells (BMMSCs), Adiposederived stem cells (ADSCs), Temperaturesensitive materials, Nonenzymatic cell recovery

摘要： 以温敏性聚 N - 异丙基丙烯酰胺( p o l y( N - i s o p r o p y l a c r y l a m i d e ) , P N I P AAm) 为主体的细胞培养基底通
过温度的改变来调控细胞在其上的黏附和脱附, 有效替代传统酶解法实现贴壁细胞的非酶解收获, 同时, 贴壁细胞
对温敏性基底具有选择性。选取贴壁型 H e L a细胞、 HEK 2 9 3细胞、 骨髓间充质干细胞( b o n em a r r o w m e s e n c h y m a l
s t e mc e l l s , BMMS C s ) 和脂肪干细胞( a d i p o s e - d e r i v e ds t e mc e l l s ,AD S C s ) 作为考察对象, 采用具有不同表面润湿性、
表面形貌和膜厚度等特征的系列P N I P AAm共聚物膜作为备选培养基底, 考察查适合这四种细胞黏附和脱附的共
聚物膜性能范围。实验结果表明, 不同种类细胞对温敏性基底具有不同的选择性, 同时为上述细胞分别选取了适宜
的温敏性共聚物膜。

关键词: 聚 N - 异丙基丙烯酰胺 , 骨髓间充质干细胞 ,  脂肪干细胞 ,  温敏性材料 ,  非酶解收获