辽宁石油化工大学学报

辽宁石油化工大学学报 ›› 2020, Vol. 40 ›› Issue (6): 14-20.DOI: 10.3969/j.issn.1672-6952.2020.06.004

• 化学与化学工程 • 上一篇    下一篇

复配菌对含蜡原油含蜡量及其黏度作用分析

孟凡怡1杜胜男1冯宪明1王超2王卫强1   

  1. 1.辽宁石油化工大学 石油天然气工程学院,辽宁 抚顺 113001; 2.中油(新疆)石油工程有限公司 设计分公司,新疆 克拉玛依 834000
  • 收稿日期:2019-12-20 修回日期:2020-03-03 出版日期:2020-12-25 发布日期:2020-12-30
  • 作者简介:孟凡怡(1995-),女,硕士研究生,从事油气储运方面的研究;E-mail:791222971@qq.com。
  • 基金资助:
    辽宁省高等学校创新人才支持计划项目(LR2018055);辽宁省自然科学基金指导计划项目(20180550745)。

Effect of Compound Bacteria on Wax Content and Viscosity of Waxy Crude Oil

Meng Fanyi1Du Shengnan1Feng Xianming1Wang Chao2Wang Weiqiang1   

  1. 1.College of Petroleum Engineering,Liaoning Shihua University,Fushun Liaoning 113001,China; 2.Design Branch of PetroChina (Xinjiang) Petroleum Engineering Co., Ltd.,Karamay Xiangjiang 834000,China
  • Received:2019-12-20 Revised:2020-03-03 Published:2020-12-25 Online:2020-12-30

摘要: 微生物能改善含蜡原油的流动性,提高运输效率。将已培养的枯草芽孢杆菌6#和铜绿假单胞菌3#按2∶3的体积比复配培养,并优化其生长条件。采用DSC、偏光显微镜、HAKEE流变仪和界面张力仪对复配菌与含蜡原油的作用效果进行分析。经复配菌处理后,含蜡原油的析蜡点降低了2.23 ℃,析蜡高峰点降低了2.71 ℃,原油含蜡量(原油中蜡质量分数)降低51.64%;蜡晶结构发生了明显变化;37 ℃下测得原油表观黏度降低了64.72%;复配菌对液体石蜡的乳化指数为55.39%,液体石蜡培养基的表面张力从79.89 mN/m 降低至38.83 mN/m。与单菌相比,复配菌在除蜡降黏方面效果更为优异,应用前景更为广阔。

关键词: 微生物降解, 生物表面活性剂, 含蜡原油, 黏度, 表面张力

Abstract: Microbes can improve the fluidity of waxy crude oil and increase the efficiency of transportation. The cultured Bacillus subtilis 6# and Pseudomonas aeruginosa 3# were mixed and cultured in a ratio of 2∶3, the growth conditions were optimized. DSC, polarizing microscope, HAKEE rheometer and interfacial tensiometer were used to analyze the effect of compound bacteria and waxy crude oil. The results showed that: after treatment with compound bacteria, the wax precipitation point of waxy crude oil decreased by 2.23 ℃, the peak point of wax precipitation decreased by 2.71 ℃, and the wax content of crude oil decreased by 51.64%; the wax crystal structure changed significantly; the apparent viscosity of crude oil decreased by 64.72% at 37 ℃. In addition, the emulsification index of the compound bacteria on liquid paraffin was 55.39%, and the surface tension of the liquid paraffin culture medium was reduced from 79.89 mN/m to 38.83 mN/m. Compared with single bacteria, compound bacteria are more effective in removing wax and reducing viscosity, and have wider application prospects.

Key words: Microbial degradation, Biosurfactant, Waxy crude oil, Viscosity, Surface tension

引用本文

孟凡怡, 杜胜男, 冯宪明, 王超, 王卫强. 复配菌对含蜡原油含蜡量及其黏度作用分析[J]. 辽宁石油化工大学学报, 2020, 40(6): 14-20.

Meng Fanyi, Du Shengnan, Feng Xianming, Wang Chao, Wang Weiqiang. Effect of Compound Bacteria on Wax Content and Viscosity of Waxy Crude Oil[J]. Journal of Liaoning Petrochemical University, 2020, 40(6): 14-20.

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