Journal of Liaoning Petrochemical University

Journal of Liaoning Petrochemical University ›› 2007, Vol. 27 ›› Issue (2): 10-12.

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Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase

  

  1. Liaoning University of Petroleum &Chemical Technology , Fushun Liaoning 113001, P .R .China
  • Received:2007-01-11 Published:2007-06-20 Online:2017-07-05

产木聚糖酶黑曲霉LN0601 的液体发酵

张 晶, 王战勇, 苏婷婷   

  1. 辽宁石油化工大学, 辽宁抚顺113001
  • 作者简介:张晶(1966 -) 女山东莒县副教授.

Abstract:

     The Aspergillus niger LN0601 was used to produce xy lanase by submerged fermentation.The influence factor s of carbon source , nitrogen source , cultural time , cultural temperature , initial pH , and inoculation volumic fraction of spore suspending liquid on the capability of the stain to produce xylanase were studied.The optimum conditions of Aspergillus niger to produce xylanase are as follows :carbon source 5 %(mass fraction)corncob powder , nitrogen source 1% (mass fraction) NaNO3 , cultural time 2 d , cultural temperature 28 ℃, initial pH 6.5, 20% inoculation volumic fraction of spore suspending liquid added to 250mL conical flask with 100mL submerged culture medium , shaking table revolution 150 r/min.Under this condition the xylanase activity could reach to mo re than 2 000U/ mL .

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Key words: Xylanase,    , Aspergillus niger,    , Submerged fermentation

摘要:      利用黑曲霉LN0601 菌株液体发酵生产木聚糖酶。分别考察培养基碳源、培养基氮源、培养时间、培养温度、培养起始pH 及孢子悬液接种体积分数等因素对该菌株产木聚糖酶性能的影响。确立了黑曲霉LN0601 产木聚糖酶的最优条件, 即以质量分数为5 %的玉米芯粉作为培养基碳源;质量分数为1 %的NaNO3 作为培养基氮源;培养时间为2 d ;培养温度为28 ℃;培养起始pH 为6.5;孢子悬液接种量体积分数为20 %, 接入装有100 mL 液体发酵培养基的250 m L 三角瓶内, 150 r/ min 振荡培养。此条件下黑曲霉LN0601 的木聚糖酶活力可达2 000 U/m L 以上。

关键词: 木聚糖酶,  黑曲霉,  液体发酵

CLC Number: 

Cite this article

ZHANG Jing,WANG Zhan -y ong,SU Ting -ting. Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase[J]. Journal of Liaoning Petrochemical University, 2007, 27(2): 10-12.

张 晶, 王战勇, 苏婷婷. 产木聚糖酶黑曲霉LN0601 的液体发酵[J]. 辽宁石油化工大学学报, 2007, 27(2): 10-12.

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