Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase

Abstract

Abstract:

　 The Aspergillus niger LN0601 was used to produce xy lanase by submerged fermentation.The influence factor s of carbon source , nitrogen source , cultural time , cultural temperature , initial pH , and inoculation volumic fraction of spore suspending liquid on the capability of the stain to produce xylanase were studied.The optimum conditions of Aspergillus niger to produce xylanase are as follows :carbon source 5 %(mass fraction)corncob powder , nitrogen source 1% (mass fraction) NaNO3 , cultural time 2 d , cultural temperature 28 ℃, initial pH 6.5, 20% inoculation volumic fraction of spore suspending liquid added to 250mL conical flask with 100mL submerged culture medium , shaking table revolution 150 r/min.Under this condition the xylanase activity could reach to mo re than 2 000U/ mL .

null

Key words: Xylanase, , Aspergillus niger, , Submerged fermentation

摘要： 　利用黑曲霉LN0601 菌株液体发酵生产木聚糖酶。分别考察培养基碳源、培养基氮源、培养时间、培养温度、培养起始pH 及孢子悬液接种体积分数等因素对该菌株产木聚糖酶性能的影响。确立了黑曲霉LN0601 产木聚糖酶的最优条件, 即以质量分数为5 %的玉米芯粉作为培养基碳源;质量分数为1 %的NaNO₃作为培养基氮源;培养时间为2 d ;培养温度为28 ℃;培养起始pH 为6.5;孢子悬液接种量体积分数为20 %, 接入装有100 mL 液体发酵培养基的250 m L 三角瓶内, 150 r/ min 振荡培养。此条件下黑曲霉LN0601 的木聚糖酶活力可达2 000 U/m L 以上。

关键词: 木聚糖酶, 　黑曲霉, 　液体发酵

CLC Number:

null

ZHANG Jing,WANG Zhan -y ong,SU Ting -ting. Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase[J]. Journal of Liaoning Petrochemical University, 2007, 27(2): 10-12.

张　晶, 王战勇, 苏婷婷. 产木聚糖酶黑曲霉LN0601 的液体发酵[J]. 辽宁石油化工大学学报, 2007, 27(2): 10-12.

[1]	Fu Danni，Li Xiaotong，Xu Canran，Huang Weiwei，Dong Xiangzhou，Zhu Qifa，Liu Yongmin，Lu Diannan. Degradation Characteristics and Mechanism of Aspergillus Niger CKB [J]. Journal of Liaoning Petrochemical University, 2019, 39(5): 13-20.
[2]	. Removal of Sulfides from Simulated Oil by CH₃CONH₂/ZnCl₂ Deep Eutectic Solvent [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 1-7.
[3]	LiuYujuan,XuJi,TongYufei,et al. Progress in Research of the Synthesis Methods of Nanometer Ceria [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 8-12.
[4]	Liu Jindong,He Xin,Xin Dingye,et al. Change Law of Four Fractions in Residue Hydrotreating Catalyst Grading System [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 13-16.
[5]	Wu Hao， Ma Guiyang， Xiang Nan，et al. The Effect Analysis of Settlement of Soil on Stress Fields of  Horizontal Section of Crossing Pipeline Structure [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 22-25.
[6]	Yan Rongju， Wang Weiqiang， Li Zimeng，et al. Progress of Severe Slugging in Marine Riser System [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 26-30.
[7]	Wang Long. Application of Seismic Reconstruction Inversion in Prediction of River  Channel of X Development Zone in Daqing Placantieline [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 31-37.
[8]	Zhu Bomin. Optimization of Well Spacing in Gaotaizi Reservoir  of Sazhong Development Zone [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 38-43.
[9]	Ma Jing,Jin Kejun,Liang Yufeng,et al. Study on Comprehensive Evaluation for Engineering  Mode of Offshore Oil and Gas Fields [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 44-48.
[10]	Fan Dengshuai,Gao Peng, Liu Zhihui，et al. A Method of Connection Reliability Analysis of Bolted Flange Systems [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 49-52.
[11]	Wu Zhiyong,Liu Yang,Gao Yang,et al. An Investigation on the Time Relaxation Parameter Used in Boiling  Simulation of Helical Coil Heat Exchanger ShellSide [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 53-60.
[12]	Gao Yang,Han Yunping,Pan Zhen,et al. Static Simulation Research and System Design of Gas Transmission Pipeline Network [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 61-66.
[13]	Fu Chun,Sun Xianglei,Wang Kun,et al. Research on Improved EMD Algorithm Based on FastICA [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 67-70.
[14]	Luan Xiuyun， Ling Xiaoqing， Yu Dan. Empirical Study on the Influence of Transformational  Leadership and Employee Innovation Behavior： The Mediators of Psychological Empowerment [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 71-75.
[15]	Li Qi， Jin Hui， Zhang Chunshu， Wang Yajun. Comprehensive Evaluation of the City Real Estate  Market Based on Factors Analysis [J]. Journal of Liaoning Petrochemical University, 2017, 37(5): 76-80.

Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase

产木聚糖酶黑曲霉LN0601 的液体发酵

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics