Separation and Identify of Oil Degradation Bacteria

Abstract

Abstract:

　Three stains (PD51 , PD53 , PD56), with good ability of degrading oil , were isolated from oil contamination soil in No . 2 Fushun Petroleum Refinery . The morphology and physio logy of three stains shows that the strain PD51 and PD53 belong to Microbacterium, while the strain PD56 belongs to Arthrobater. After the sestrains was cultivated at 28 ℃ for 72 h , the biodegradable ratios of PD51 , PD53 and PD56 for petroleum hydro carbon were 76. 63 %, 76. 47 % and 76. 17%, respectively. The biodegradable ratio of the mixed strains reaches to 84. 31 %. The results show that the mixed stains exhibit better biodegrading ability for oil than that of the single strain. And the stain PD56 has the ability of degrading phenol and phenanthrene.

Key words: 　Oil , , Degradation, , Microbacterium , , Arthrobater

摘要： 从抚顺石油二厂厂区石油污染土壤中筛选出3 株具有较强石油降解能力的菌株PD51 , PD53 , PD56。通过形态学观察和生理生化指标对这3 种微生物进行鉴定, 初步确定菌株PD51 和PD53 属于微杆菌属(Microbacterium), 而菌株PD56 属于节杆菌属(Arthrobater)。菌株在28 ℃培养72 h 后, 发现这3 株菌株(PD51,PD53, PD56)对石油烃的降解率分别为76. 63 %, 76. 47 %, 76. 17 %。而这3 株菌株的混合菌株对石油烃的降解率达到了84. 31%。结果表明, 混合菌株对石油烃的降解能力要优于单一菌株对石油烃的降解能力。实验中同时发现菌株PD56 还具有利用苯酚和菲等芳烃的能力。

关键词: 石油, 　降解, 　微杆菌属, 　节杆菌属

CLC Number:

null

GU Gui - zho u,WANG Zhan - yo ng,YU Yong,SUN Da - qi. Separation and Identify of Oil Degradation Bacteria[J]. Journal of Liaoning Petrochemical University, 2007, 27(1): 24-26.

顾贵洲, 王战勇, 于　泳, 孙大琦. 石油降解菌株的筛选及鉴定[J]. 辽宁石油化工大学学报, 2007, 27(1): 24-26.

[1]	Kuo LI, Hui WANG, Shengnan DU, Weiqiang WANG. Optimization of Degradation Conditions of Paraffin⁃Degrading Mixed Bacteria Based on Orthogonal Experiment and Response Surface Methodology [J]. Journal of Liaoning Petrochemical University, 2024, 44(5): 22-30.
[2]	Hongjiang RUAN, Qiaoliang ZHANG, Feng LIU, Ruibin LU, Zhenni CHEN, Jiefang ZHANG, Chang WU. Physical Simulation Experiment on CO₂ Storage Mechanism in Porous Media [J]. Journal of Liaoning Petrochemical University, 2024, 44(2): 36-41.
[3]	Zheyu PANG, Jingjing ZHAO, Tianli WAN, Jiajun LI, Lingsen YANG, Siyu ZHOU, Tingting SU. Screening, Identification and Degradation Characteristic of Polystyrene Degrading Bacteria [J]. Journal of Liaoning Petrochemical University, 2024, 44(1): 15-20.
[4]	Wenbo LIU, Bowen SHI, Yaming QI, Xiaoyu LIU, Zhihua WANG. Mechanism of Wax Deposition on Wall of Waxy Condensates under the Synergistic Effect of Complex Components [J]. Journal of Liaoning Petrochemical University, 2023, 43(6): 1-10.
[5]	Dong HAN, Ping WANG, Lei TANG, Lu ZHANG, Baogang LI, Farong YANG, Xudong ZHANG. Gas Injection Compatibility and Mixing Mechanism in Bohai Heavy Oil Field [J]. Journal of Liaoning Petrochemical University, 2023, 43(5): 42-49.
[6]	Jiacong WANG, Peng GAO, Junxiu CHANG, Siqi TIAN, Yonghai CUI. Time⁃Varying Reliability Analysis of Mechanisms Considering Wear Degradation under Small Samples [J]. Journal of Liaoning Petrochemical University, 2023, 43(5): 56-60.
[7]	Xiangyu Ren, Baokuan Chen, Jing Sun, Yanfeng Bi. Double Polyoxometalates⁃Based Metal⁃Organic Framework with Photocatalytic Degradation Properties [J]. Journal of Liaoning Petrochemical University, 2023, 43(4): 51-58.
[8]	Xiangdong Jiang, Zhaohui Zhou, Lu Zhang, Guiyang Ma. Study on the Mechanism of the Novel Extended Surfactants on Reducing the Interfacial Tensions [J]. Journal of Liaoning Petrochemical University, 2023, 43(3): 47-51.
[9]	Donghai Yang, Xiaorui Cheng, Jian Luan, Hongxu Zhong, Chaohui Chen, Xinming Zhao, Weihong Xie, Qing Yun. Investigation on Optimization of Parameters in Treating Dagang Shale Oil under High Frequency Electric Field [J]. Journal of Liaoning Petrochemical University, 2023, 43(2): 1-6.
[10]	Fuqiang Zhang, Kun Wang, Lijuan Gao, Duoqi Wang, Qiyu Huang. Effect of Interface Characteristics of FRP Pipe Wall/Crude Oil on Low⁃Temperature Transportation of High Water⁃Cut Crude Oil [J]. Journal of Liaoning Petrochemical University, 2023, 43(2): 42-46.
[11]	Shu'nan Hei, Kun Wang, Chunxing Zheng, Jiancheng Miao, Qiyu Huang. Research on Determination Method of Low Temperature Gathering and Transportation Temperature of Dissolved Gas Crude Oil with High Water Cut [J]. Journal of Liaoning Petrochemical University, 2023, 43(1): 32-37.
[12]	Changhua Lin, Yang Li, Hongwei Chen, Weiqiang Qian, Lianjun Deng. Design and Thermodynamic Performance Analysis of Solar Heating Crude Oil System [J]. Journal of Liaoning Petrochemical University, 2023, 43(1): 43-48.
[13]	Baoren Wang, Tingting Su, Zhanyong Wang. A Strain Degrading Nonionic Surfactant Triton X⁃100：Screening and Its Optimization of Degradation Conditions [J]. Journal of Liaoning Petrochemical University, 2022, 42(6): 16-20.
[14]	Shoude Wang, Dejun Liu, Liao Ma. Research Progress of Chemical Wax Inhibitors for Waxy Crude Oil Pipelines [J]. Journal of Liaoning Petrochemical University, 2022, 42(6): 8-15.
[15]	Yiming Wu, Kun Yao, Yan Liu, Xiangyun Li, Mimi Wu, Ronghong Cheng. Study on Numerical Simulation of Gas Injection for Enhanced Oil Recovery in Condensate Gas Reservoir：Take the Yaha Gas Reservoir as an Example [J]. Journal of Liaoning Petrochemical University, 2022, 42(3): 51-55.

Separation and Identify of Oil Degradation Bacteria

石油降解菌株的筛选及鉴定

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics