Extraction of Flavonoids from Ligusticum and Its Antioxidant Activity in Vitro

doi:10.12422/j.issn.1672-6952.2023.02.004

Abstract

Abstract:

In this paper, the extraction process of flavonoids from Ligusticum was optimized by single factor test and response surface methodology, and the antioxidant experiment in vitro was carried out. The optimum extraction conditions were as follows: extraction time 2.5 h, extraction temperature 83 ℃, ethanol concentration 84%, liquid?solid ratio 35 L/g (the ratio of ethanol volume to Ligusticum jeholense powder sample mass). Under these conditions, the yield of flavonoids from Ligusticum was （22.64±0.18） mg/g. The results of antioxidant experiment in vitro showed that flavonoids from Ligusticum had certain antioxidant capacity. The clearing effect of ·DPPH is the best, the clearing effect of ·OH is the second, and the clearing effect of·O^2- is the worst. It also has the ability to restore Fe³⁺.

Key words: Ligusticum, Flavonoids, Extraction, Antioxidant activity

摘要：

采用单因素实验与响应面实验结合的方法，优化了藁本中黄酮类化合物的提取工艺，并进行了体外抗氧化实验。结果表明，最佳提取工艺条件为：提取时间为2.5 h，提取温度为83 ℃，乙醇体积分数为84%，液料比为35 L/g（乙醇溶液体积与藁本粉末样品质量之比）；在最佳提取条件下，藁本黄酮得率达到（22.64±0.18） mg/g；藁本黄酮具有一定的抗氧化能力，对·DPPH的清除效果最好，对·OH的清除效果次之，对·O $2 -$ 的清除效果最差；对Fe³⁺具有一定的还原能力。

关键词: 藁本, 黄酮, 提取, 抗氧化活性

CLC Number:

TQ911

Yajun Feng, Qiuju Han, Chen Wang, Ruiyue Pan, Lu Ren, Jingwen Luo. Extraction of Flavonoids from Ligusticum and Its Antioxidant Activity in Vitro[J]. Journal of Liaoning Petrochemical University, 2023, 43(2): 20-25.

冯雅郡, 韩秋菊, 王晨, 潘睿悦, 任璐, 罗静雯. 藁本中黄酮类化合物的提取及体外抗氧化活性研究[J]. 辽宁石油化工大学学报, 2023, 43(2): 20-25.

Figures/Tables 11

水平	A（提取时间/h）	B（提取温度/℃）	C（乙醇体积分数/%）	D[液料比/（L·g^－1）]
1	1.5	70	70	25
0	2.0	80	80	30
－1	2.5	90	90	35

实验编号	A	B	C	D	黄酮得率/ （mg·g^－1）	实验编号	A	B	C	D	黄酮得率/ （mg·g^－1）
1	－1	－1	0	0	17.69	5	0	0	－1	－1	15.76
2	1	－1	0	0	19.45	6	0	0	1	－1	16.93
3	－1	1	0	0	18.18	7	0	0	－1	1	18.96
4	1	1	0	0	21.35	8	0	0	1	1	19.92
9	－1	0	0	－1	15.92	20	1	0	1	0	21.94
10	1	0	0	－1	21.07	21	0	－1	0	－1	17.31
11	－1	0	0	1	18.07	22	0	1	0	－1	18.54
12	1	0	0	1	22.76	23	0	－1	0	1	19.38
13	0	－1	－1	0	16.31	24	0	1	0	1	18.30
14	0	1	－1	0	17.26	25	0	0	0	0	18.73
15	0	－1	1	0	17.60	26	0	0	0	0	18.76
16	0	1	1	0	19.31	27	0	0	0	0	19.96
17	－1	0	－1	0	16.03	28	0	0	0	0	18.94
18	1	0	－1	0	20.23	29	0	0	0	0	19.00
19	－1	0	1	0	18.16

方差来源	SS	DF	MS	F值	P值
R²=0.907		R $A d j 2$ =0.814		CV=4.05%
模型	78.340	14	5.600	9.750	< 0.000 1^**
A	43.130	1	43.130	75.160	< 0.000 1^**
B	2.250	1	2.250	3.930	0.067 5
C	7.220	1	7.220	12.590	0.003 2^**
D	11.720	1	11.720	20.430	0.000 5^**
AB	0.500	1	0.500	0.870	0.367 8
AC	0.044	1	0.044	0.077	0.785 7
AD	0.053	1	0.053	0.092	0.765 9
BC	0.140	1	0.140	0.250	0.623 7
BD	1.330	1	1.330	2.320	0.149 6
CD	0.011	1	0.011	0.019	0.891 7
A²	3.330	1	3.330	5.800	0.030 4^*
B²	2.000	1	2.000	3.490	0.083 0
C²	4.570	1	4.570	7.960	0.013 6^*
D²	0.490	1	0.490	0.860	0.370 4
残差	8.030	14	0.570
失拟度	7.010	10	0.700	2.730	0.172 3
纯误差	1.030	4	0.260
总离差	86.370	28

方差来源	SS	DF	MS	F值	P值
R²=0.907		R $A d j 2$ =0.814		CV=4.05%
模型	78.340	14	5.600	9.750	< 0.000 1^**
A	43.130	1	43.130	75.160	< 0.000 1^**
B	2.250	1	2.250	3.930	0.067 5
C	7.220	1	7.220	12.590	0.003 2^**
D	11.720	1	11.720	20.430	0.000 5^**
AB	0.500	1	0.500	0.870	0.367 8
AC	0.044	1	0.044	0.077	0.785 7
AD	0.053	1	0.053	0.092	0.765 9
BC	0.140	1	0.140	0.250	0.623 7
BD	1.330	1	1.330	2.320	0.149 6
CD	0.011	1	0.011	0.019	0.891 7
A²	3.330	1	3.330	5.800	0.030 4^*
B²	2.000	1	2.000	3.490	0.083 0
C²	4.570	1	4.570	7.960	0.013 6^*
D²	0.490	1	0.490	0.860	0.370 4
残差	8.030	14	0.570
失拟度	7.010	10	0.700	2.730	0.172 3
纯误差	1.030	4	0.260
总离差	86.370	28

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