Abstract: The separation and purification conditions of total flavonoids from Flos Lonicerae were studied by polyamide column chromatography. Based on the concentration of the total flavonoids, by the way of the adsorption and desorption abilities of polyamide resin, the optimum parameters of the separation and purification of the total flavonoids of Hibiscus manihot L are selected and evaluated. The main results are as follows: Purification conditions are as follows: the concentration of sample solution is 4.68 mg/mL,the pH is 4~5, adsorption rare is 1.5 mL/min, sample volume is 1.5 BV, water washing resin column with 3.0 BV, the elution rate is 1.5 mL/min with 5.0 BV 80% ethanol has a best effect with polyamides for five times. The result provided a basis for further development andapplication of Hibiscus manihot L.

Key words: Hibiscus manihot L. flower, Total flavonoids, Polyamides, Purification

摘要：   利用聚酰胺柱层析对金花葵花总黄酮的分离纯化条件进行了研究。以金花葵花总黄酮质量浓度为指标，通过考察聚酰胺树脂对黄酮的吸附和解吸能力，筛选出聚酰胺柱层析分离纯化金花葵花总黄酮的最优参数。结果表明，当金花葵花总黄酮的上样液质量浓度为4.68 mg/mL、pH为4~5，在1.5 mL/min的吸附流速下上样1.5 BV时，可达到吸附饱和。接下来用3.0 BV水淋洗去杂质，再用5.0 BV 体积分数为80%乙醇解吸液，在1.5 mL/min的解吸流速下进行解吸，树脂每使用5次需要进行一次再生处理，此时分离纯化效果最佳。该研究结果为金花葵花的深入开发和应用提供了依据。

关键词: 金花葵花, 　总黄酮, 　聚酰胺, 　纯化