阴沟肠杆菌分泌生物表面活性剂的合成研究

辽宁石油化工大学学报 ›› 2008, Vol. 28 ›› Issue (3): 8-11.

阴沟肠杆菌分泌生物表面活性剂的合成研究

徐圆圆1，张坤2，花秀夫2，王君2，刘永民1*，刘铮2

1. 辽宁石油化工大学石油化工学院，辽宁抚顺 113001|2.清华大学化工系，北京 100084

收稿日期:2008-04-14 出版日期:2008-09-20 发布日期:2017-07-24
基金资助:
中国石油化工股份有限公司科学技术研究开发项目（306038）。

Production of Biosurfactant by Enterobacter Cloacae and Its Effect on an Enhanced Water-Solubility of Phenanthrene

XU Yuan-yuan1, ZHANG Kun2, HUA Xiu-Fu2, WANG Jun2, LIU Yong-min1*, LIU Zheng2

1.School of Petrochemical Engineering, Liaoning University of Petroleum & Chemical Technology, Fushun Liaoning 113001, P.R.China; 2.Department of Chemical Engineering, Tsinghua University, Beijing 100084, P.R.China

Received:2008-04-14 Published:2008-09-20 Online:2017-07-24

摘要/Abstract

摘要： 对阴沟肠杆菌（Enterobacter cloacae）分泌表面活性剂的行为进行了初步研究，结果表明E.cloacae能以葡萄糖等水溶性碳源产生糖脂类生物表面活性剂，以葡萄糖为碳源、氯化铵为氮源时其最佳培养基组成的质量分数分别是3%、0.1%，碳氮比为8.9，初始pH值为7.2。该生物表面活性剂对菲有较强的增溶效果，并可强化高岭土所吸附的菲的脱附，可望促进石油烃污染土壤的生物修复过程。

关键词: 阴沟肠杆菌, 生物表面活性剂, 糖脂, 菲

Abstract: A glycolipids type biosurfactant, as identified by FTIR was obtained from Enterobacter cloacae, a petroleum-degrading bacterium. The optimum productivity of the biosurfactant was obtained. An optimal yield of biosurfactant was achieved at a n(C)/n(N) ratio of 8.9 using glucose as carbon source and ammonium chloride as nitrogen source when the initial pH value of the culture media is 7.2. It is shown that the presence of the biosurfactant greatly enhanced water-solubility of phenanthrene and the removal of phenanthrene from kaolinite soil. These would thus improve the bioavailability of phenanthrene and intensify the bioremediation of petroleum contaminated soil.

Key words: Enterobacter cloacae, Biosurfactant, Glycolipids, Phenanthrene

徐圆圆，张坤，花秀夫，王君，刘永民，刘铮. 阴沟肠杆菌分泌生物表面活性剂的合成研究[J]. 辽宁石油化工大学学报, 2008, 28(3): 8-11.

XU Yuan-yuan, ZHANG Kun, HUA Xiu-Fu, WANG Jun, LIU Yong-min, LIU Zheng. Production of Biosurfactant by Enterobacter Cloacae and Its Effect on an Enhanced Water-Solubility of Phenanthrene[J]. Journal of Liaoning Petrochemical University, 2008, 28(3): 8-11.

[1]	冯宪明, 周金喜, 王邻睦, 陈建宝. 含油污泥热化学清洗剂研究进展[J]. 辽宁石油化工大学学报, 2023, 43(5): 7-13.
[2]	孟凡怡，杜胜男，冯宪明，王超，王卫强. 复配菌对含蜡原油含蜡量及其黏度作用分析[J]. 辽宁石油化工大学学报, 2020, 40(6): 14-20.
[3]	宁春莹,李政,顾贵洲,杨磊. 糖脂类生物表面活性剂在采油中的应用[J]. 辽宁石油化工大学学报, 2016, 36(2): 13-16.