产木聚糖酶黑曲霉LN0601 的液体发酵

辽宁石油化工大学学报 ›› 2007, Vol. 27 ›› Issue (2): 10-12.

产木聚糖酶黑曲霉LN0601 的液体发酵

张　晶, 王战勇, 苏婷婷

辽宁石油化工大学, 辽宁抚顺113001

收稿日期:2007-01-11 出版日期:2007-06-20 发布日期:2017-07-05
作者简介:张晶(1966 -) 女山东莒县副教授.

Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase

Liaoning University of Petroleum &Chemical Technology , Fushun Liaoning 113001, P .R .China

Received:2007-01-11 Published:2007-06-20 Online:2017-07-05

摘要/Abstract

摘要： 　利用黑曲霉LN0601 菌株液体发酵生产木聚糖酶。分别考察培养基碳源、培养基氮源、培养时间、培养温度、培养起始pH 及孢子悬液接种体积分数等因素对该菌株产木聚糖酶性能的影响。确立了黑曲霉LN0601 产木聚糖酶的最优条件, 即以质量分数为5 %的玉米芯粉作为培养基碳源;质量分数为1 %的NaNO₃作为培养基氮源;培养时间为2 d ;培养温度为28 ℃;培养起始pH 为6.5;孢子悬液接种量体积分数为20 %, 接入装有100 mL 液体发酵培养基的250 m L 三角瓶内, 150 r/ min 振荡培养。此条件下黑曲霉LN0601 的木聚糖酶活力可达2 000 U/m L 以上。

null

关键词: 木聚糖酶, 　黑曲霉, 　液体发酵

Abstract:

　 The Aspergillus niger LN0601 was used to produce xy lanase by submerged fermentation.The influence factor s of carbon source , nitrogen source , cultural time , cultural temperature , initial pH , and inoculation volumic fraction of spore suspending liquid on the capability of the stain to produce xylanase were studied.The optimum conditions of Aspergillus niger to produce xylanase are as follows :carbon source 5 %(mass fraction)corncob powder , nitrogen source 1% (mass fraction) NaNO3 , cultural time 2 d , cultural temperature 28 ℃, initial pH 6.5, 20% inoculation volumic fraction of spore suspending liquid added to 250mL conical flask with 100mL submerged culture medium , shaking table revolution 150 r/min.Under this condition the xylanase activity could reach to mo re than 2 000U/ mL .

Key words: Xylanase, , Aspergillus niger, , Submerged fermentation

中图分类号:

null

张　晶, 王战勇, 苏婷婷. 产木聚糖酶黑曲霉LN0601 的液体发酵[J]. 辽宁石油化工大学学报, 2007, 27(2): 10-12.

ZHANG Jing,WANG Zhan -y ong,SU Ting -ting. Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase[J]. Journal of Liaoning Petrochemical University, 2007, 27(2): 10-12.

[1]	关转飞,贾飞,马学良,杨翔华. GJY-08菌株产木聚糖酶的固体发酵条件的研究[J]. 辽宁石油化工大学学报, 2011, 31(3): 8-11.
[2]	王战勇, 苏婷婷. 产纤维素酶黑曲霉LN0401 液体发酵条件的分析[J]. 辽宁石油化工大学学报, 2006, 26(2): 27-30.