秸秆降解菌株CKB的降解特性与机理

doi:10.3969/j.issn.1672-6952.2019.05.003

辽宁石油化工大学学报 ›› 2019, Vol. 39 ›› Issue (5): 13-20.DOI: 10.3969/j.issn.1672-6952.2019.05.003

秸秆降解菌株CKB的降解特性与机理

付丹妮^1,2，李晓桐²，徐粲然²，黄魏魏³，董祥洲³，朱启法³，刘永民¹，卢滇楠²

（1.辽宁石油化工大学化学化工与环境学部，辽宁抚顺 113001； 2.清华大学化学工程系，北京 100084； 3.安徽皖南烟叶责任有限公司，安徽宣城 242000）

收稿日期:2018-05-05 修回日期:2018-05-31 出版日期:2019-10-31 发布日期:2019-10-30
通讯作者: 刘永民（1959⁃），男，教授，从事化学工程方面的研究；E⁃mail：liu79ym@tom.com。
作者简介:付丹妮（1992-），女，硕士研究生，从事化学工程方面的研究；E-mail：fdn19921118@163.com
基金资助:
国家自然科学基金项目（21476125）

Degradation Characteristics and Mechanism of Aspergillus Niger CKB

Fu Danni^1,2，Li Xiaotong²，Xu Canran²，Huang Weiwei³，Dong Xiangzhou³，Zhu Qifa³，Liu Yongmin¹，Lu Diannan²

(1.College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun Liaoning 113001, China； 2.Department of Chemical Engineering, Tsinghua University, Beijing 100084, China； 3.Anhui Wannan Tobacco Liability Company, Xuancheng Anhui 242000, China）

Received:2018-05-05 Revised:2018-05-31 Published:2019-10-31 Online:2019-10-30

摘要/Abstract

摘要： 从常年水稻种植土壤中筛选出1株能够高效降解秸秆的菌株CKB，经过ITS序列分析鉴定为黑曲霉（Aspergillus niger）。在常温正常土壤环境中，秸秆经该菌35 d降解，失重率可达49%。进一步考察了CKB对于纤维素和木质素的降解能力，72 h时纤维素转化葡萄糖质量浓度为0.554 g/L，木质素降解量达到0.607 g/L；同时也考察了不同底物质量浓度、pH、温度对于纤维素和木质素降解效果的影响。通过SEM观察秸秆降解前后的结构变化，并利用Pyrolysis⁃GC/MS手段对降解产物进行检测与表征，证明了秸秆腐化变黑产物为腐殖酸的经典组分,且CKB在低温环境也有良好的降解效果，阐释了黑曲霉CKB的秸秆降解机理。

关键词: 黑曲霉, 秸秆降解, 降解机理, 木质素降解, 纤维素降解

Abstract: A strain named of CKB that can efficiently degrade straw was selected from soil where growing rice perennially and which was identified as Aspergillus niger by ITS sequence analysis. In the normal soil temperature and environment, the straw was degraded by the bacteria for 35 days, and the weight loss rate reached 49%. Furthermore, the degradability of cellulose and lignin by CKB was also investigated. At 72 h of the degradation, the converted glucose concentration from cellulose was 0.554 g/L, while the amount of lignin degradation was 0.607 g/L. The effect of substrate concentration, pH, and temperature on the degradation of cellulose and lignin was investigated. The structural change of straw before and after degradation were observed by SEM. The products of degradation were detected and characterized by means of Pyrolysis⁃GC/MS; the degradation mechanism of Aspergillus niger CKB was explained.

Key words: Aspergillus niger, Straw degradation, Mechanism of degradation, Degradation of lignin, Degradation of cellulose

付丹妮，李晓桐，徐粲然，黄魏魏，董祥洲，朱启法，刘永民，卢滇楠. 秸秆降解菌株CKB的降解特性与机理[J]. 辽宁石油化工大学学报, 2019, 39(5): 13-20.

Fu Danni，Li Xiaotong，Xu Canran，Huang Weiwei，Dong Xiangzhou，Zhu Qifa，Liu Yongmin，Lu Diannan. Degradation Characteristics and Mechanism of Aspergillus Niger CKB[J]. Journal of Liaoning Petrochemical University, 2019, 39(5): 13-20.

[1]	张　晶, 王战勇, 苏婷婷. 产木聚糖酶黑曲霉LN0601 的液体发酵[J]. 辽宁石油化工大学学报, 2007, 27(2): 10-12.
[2]	王战勇, 苏婷婷. 产纤维素酶黑曲霉LN0401 液体发酵条件的分析[J]. 辽宁石油化工大学学报, 2006, 26(2): 27-30.

秸秆降解菌株CKB的降解特性与机理

Degradation Characteristics and Mechanism of Aspergillus Niger CKB

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