In order to evaluate the influence of the construction parameters of the plugging agent on the plugging removal effect, the plugging sequence and the displacement optimization of the polymer flooding wells were studied with the plugging removal rate as the evaluation index. The core experiment was carried out through the displacement of injection plugging agent and the injection order of three main chemicals, and the best construction parameters (including the best displacement and the injection sequence) were obtained. Experimental results showed that polymer injection well plugging agent were higher than 70% by changing injection order of chemical. When the organic scale corrosion agent, polymer degradation agent and solid blocking agent were injected sequentially, the plugging removal rate was the highest, and the plugging removal effect was better than other injection combinations. For the permeability of core 300 mD, when the displacement of plugging agent is larger than 0.1 mL/min, the displacement had little influence on the plugging removal effect, and the blockage removal rate was above 90%. In order to speed up the construction schedule and shorten the cycle, large displacement injection should be used. when the core permeability is 100 mD, plugging rate was less than 90%. With the increase of displacement, the rate of plug removal decreased. In order to achieve good plugging removal effect, it was advisable to choose the lower injection displacement. The higher the permeability, the better the final solution. The plugging effect of polymer flooding can be further improved by optimizing the injection sequence and displacement of the plugging agent, which can provide the basis for improving the quality of plug removal.