Journal of Petrochemical Universities

Journal of Petrochemical Universities ›› 2009, Vol. 22 ›› Issue (3): 33-36.DOI: 10.3696/j.issn.1006-396X.2009.03.009

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Isolation and Characterization of Effective Biosurfactant－Producing Bacteria Lz２－１

  

  1. Faculty of Chemical Science & Engineering, China University of Petroleum, Beijing 102249，P.R.China
  • Received:2008-06-06 Revised:2009-04-17 Published:2009-09-25 Online:2009-09-25

高效产表面活性剂菌株(Lz2-1)的筛选及其特性研究

王 靖,章厚名,安明泉,陈 云,刘洁丽,郝以周   

  1. 中国石油大学（北京）化学科学与工程学院，北京102249
  • 作者简介:王靖(1954 -), 女, 北京市, 教授, 博士
  • 基金资助:
    国家863 项目(2007AA021306);中国石油天然气股份有限公司科技开发项目(2008A-1403)。

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Abstract:

A strain Lz2-1 capable of crude oil as main source and energy of producing biosurfactants could be isolated by a series of steps including hydrocarbon enrichment culture and shaking culture and oil displacement activity assay etc. The strain was isolated with the best surface activity in 24 samples from petroleum-contained soil, sludge, waste water etc,and identified as pseudomonas aeruginosa by analysis of 16S rDNA gene and physiochemical methods. The strain produced crude biosurfactant has excellent emulsification property and makes the surface tension of water decrease from 72 mN/m to 28 mN/m. The key components of the crude biosurfactant were extracted and identified as rhamnolipid species by using TLC and FT-IR analyses. The critical micelle concentration of the biosurfactants is 0.630 47 g/L. The present study states that the biosurfactants are major factors of Lz2-1 strain in microbial enhanced oil recovery.

Key words: Biosurfactant , Pseudomonas aeruginosa , Surface tension , Rhamnolipid

摘要： 从24份含油土壤和水等样品中，经富集培养、摇瓶培养和排油活性测定等方法筛选出一株能以原油为碳源产生表面活性剂的菌株Lz2-1；该菌株可以将水的表面张力由72 mN/m降到28 mN/ m，且发酵液具有较好的乳化活性；经生理生化 16S rDNA及生理生化实验确定该菌株为铜绿假单胞菌；提取其代谢产物经薄层层析及红外色谱分析显示，主要的表面活性剂类物质为鼠李糖脂类，其临界胶束质量浓度为0 .630 47 g/L。结果表明，表面活性物质是 Lz2-1菌株在微生物采油过程中发挥作用的主要因素。

关键词: 生物表面活性剂 , 铜绿假单胞菌 , 表面张力 , 鼠李糖脂

Cite this article

WANG Jing, ZHANG Hou-ming, AN Ming-quan, et al. Isolation and Characterization of Effective Biosurfactant－Producing Bacteria Lz２－１[J]. Journal of Petrochemical Universities, 2009, 22(3): 33-36.

王 靖,章厚名,安明泉. 高效产表面活性剂菌株(Lz2-1)的筛选及其特性研究[J]. 石油化工高等学校学报, 2009, 22(3): 33-36.

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