The basic nitrogen-compounds were removed with complexation by denitrification reagent form the Fushun shale oil. The effect of reaction temperature, reaction time, volume ratio of agent to oil, complexing agent amount and settling time on the denitrification ratio and yield were investigated. The optimum operating conditions are as follows: The temperature 55~60 ℃, reaction time 5 min, volume ratio agent and oil 0.07, auxiliary usage 1∶0.5～1∶1, motionless time 35 min. Under these conditions, the denitrification ratio and oil yield can reach 95.36% and 91.25%, respectively. The results show that mixed solvent could be used in denitrification with high efficiency, pH of the shale oil dealed with reagent B is neutral.