LabVIEW is a graphical programming language, which is widely used by industry and laboratory. RBF neural network is a three layer feedforward network, which can effectively carry out non-linear model identification. Three types of RBF neural network calculation methods implemented in LabVIEW were introduced. It completed with LabVIEW RBF neural network program development and verification by the function approximation. The simulation results show that the RBF neural network combined with the virtual instrument development platform broaden its applications.