A Strain Degrading Nonionic Surfactant Triton X⁃100：Screening and Its Optimization of Degradation Conditions

doi:10.3969/j.issn.1672-6952.2022.06.003

Journal of Liaoning Petrochemical University ›› 2022, Vol. 42 ›› Issue (6): 16-20.DOI: 10.3969/j.issn.1672-6952.2022.06.003

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A Strain Degrading Nonionic Surfactant Triton X⁃100：Screening and Its Optimization of Degradation Conditions

Baoren Wang¹(), Tingting Su¹(), Zhanyong Wang²

^1.School of Petrochemical Engineering，Liaoning Petrochemical University，Fushun Liaoning 113001，China
^2.College of Bioscience and Biotechnology，Shenyang Agricultural University，Shenyang Liaoning 110866，China

Received:2021-04-25 Revised:2021-05-20 Published:2022-12-25 Online:2023-01-07
Contact: Tingting Su

一株Triton X⁃100降解菌的分离鉴定及降解条件优化

王宝任¹(), 苏婷婷¹(), 王战勇²

^1.辽宁石油化工大学石油化工学院，辽宁抚顺 113001
^2.沈阳农业大学生物科学技术学院，辽宁沈阳 110866

通讯作者: 苏婷婷
作者简介:王宝任（1995⁃），男，硕士研究生，从事环境友好型高分子材料的生物降解研究；E⁃mail：1284502060@qq.com。
基金资助:
辽宁省兴辽英才计划青年拔尖人才项目(XLYC1807034)

Abstract

Abstract:

A strain ISB5 with the ability to degrade the non?ionic surfactant Triton X?100 was screened from the activated sludge in the aerobic aeration tank of a sewage treatment plant in a chemical plant. Through sequencing and comparison analysis of the 16S rDNA of the strain, the strain ISB5 was identified as Pseudomonas sp. The culture conditions of strain ISB5 to degrade Triton X?100 were further investigated, and the suitable degradation conditions of the strain were obtained: Culture temperature is 30 ℃, initial concentration of Triton X?100 is 4 g/L, culture time is 28 h, shaking rate is 150 r/min, filling volume is 100.00 mL, inoculation the amount is 1.00% (volume ratio of strain ISB5 to medium). The degradation rate of TritonX?100 by the strain under this condition can reach more than 93.0%.

Key words: Triton X?100, Biodegradation, Degradation rate

摘要：

从某化工厂污水处理车间好氧曝气池的活性污泥中，筛选了一株对非离子表面活性剂Triton X?100具有降解能力的菌株ISB5。对菌株的16S rDNA进行测序和比对分析，将菌株ISB5鉴定为假单胞菌属（Pseudomonas sp.）。进一步对菌株ISB5降解Triton X?100的培养条件进行考察，得到了菌株的适宜降解条件：培养温度为30 ℃，Triton X?100的初始质量浓度为4 g/L，培养时间为28 h，振荡速率为150 r/min，装液量为100.00 mL，接种量为1.00%（菌株ISB5与培养基的体积比）。在该条件下，TritonX?100的降解率可达93.0%以上。

关键词: Triton X?100, 生物降解, 降解率

CLC Number:

TQ085

Baoren Wang, Tingting Su, Zhanyong Wang. A Strain Degrading Nonionic Surfactant Triton X⁃100：Screening and Its Optimization of Degradation Conditions[J]. Journal of Liaoning Petrochemical University, 2022, 42(6): 16-20.

王宝任, 苏婷婷, 王战勇. 一株Triton X⁃100降解菌的分离鉴定及降解条件优化[J]. 辽宁石油化工大学学报, 2022, 42(6): 16-20.

Figures/Tables 8

References 12

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A Strain Degrading Nonionic Surfactant Triton X⁃100：Screening and Its Optimization of Degradation Conditions

一株Triton X⁃100降解菌的分离鉴定及降解条件优化

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