适宜不同种类细胞的温敏性共聚物膜的筛选

doi:10.3696/j.issn.1672-6952.2014.03.004

辽宁石油化工大学学报 ›› 2014, Vol. 34 ›› Issue (3): 14-18.DOI: 10.3696/j.issn.1672-6952.2014.03.004

适宜不同种类细胞的温敏性共聚物膜的筛选

杨磊¹,王战勇¹,范晓光²

( 1. 辽宁石油化工大学环境与生物工程学院, 辽宁抚顺1 1 3 0 0 1; 2. 中国石油抚顺石化分公司, 辽宁抚顺1 1 3 0 0 8)

收稿日期:2013-11-15 修回日期:2014-03-02 出版日期:2014-06-25 发布日期:2017-07-14
作者简介:杨磊(1982-),女,博士,讲师,从事生物材料的研究;E-mail:LeiYang1982@yahoo.com。
基金资助:
国家自然科学基金项目(31100099);辽宁石油化工大学科学基金项目(80040139)。

Selection of TemperatureResponsive Copolymer Film for Different Cells

Yang Lei1，Wang Zhanyong1，Fan Xiaoguang2

（1.School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun Liaoning 113001,China; 2.Fushun Petrochemical Company, PetroChina Company Limited, Fushun Liaoning 113008, China）

Received:2013-11-15 Revised:2014-03-02 Published:2014-06-25 Online:2017-07-14

摘要/Abstract

摘要： 以温敏性聚 N - 异丙基丙烯酰胺( p o l y( N - i s o p r o p y l a c r y l a m i d e ) , P N I P AAm) 为主体的细胞培养基底通
过温度的改变来调控细胞在其上的黏附和脱附, 有效替代传统酶解法实现贴壁细胞的非酶解收获, 同时, 贴壁细胞
对温敏性基底具有选择性。选取贴壁型 H e L a细胞、 HEK 2 9 3细胞、骨髓间充质干细胞( b o n em a r r o w m e s e n c h y m a l
s t e mc e l l s , BMMS C s ) 和脂肪干细胞( a d i p o s e - d e r i v e ds t e mc e l l s ,AD S C s ) 作为考察对象, 采用具有不同表面润湿性、
表面形貌和膜厚度等特征的系列P N I P AAm共聚物膜作为备选培养基底, 考察查适合这四种细胞黏附和脱附的共
聚物膜性能范围。实验结果表明, 不同种类细胞对温敏性基底具有不同的选择性, 同时为上述细胞分别选取了适宜
的温敏性共聚物膜。

关键词: 聚 N - 异丙基丙烯酰胺 , 骨髓间充质干细胞 , 脂肪干细胞 , 温敏性材料 , 非酶解收获

Abstract: Temperatureresponsive culture substrates containing poly(Nisopropylacrylamide) (PNIPAAm) have been developed as an effective substitute for enzymatic treatment to recover adherent cells, which can control cell attachment and detachment by changing temperature. However, not all PNIPAAmcontaining surfaces can be applicable to cell culture and recovery. That means adherent cells show accommodative selectivity for thermoresponsive substrates. In this study, different cells including HeLa cells, HEK293 cells, bone marrow mesenchymal stem cells (BMMSCs) and adiposederived stem cells (ADSCs) were cultured on the temperatureresponsive PNIPAAm copolymer films with different surface wettability, topography and film thickness. The experimental results demonstrate that various cells had their special thermoresponsive substrates, and the optimal copolymer films were selected for HeLa cells, HEK293 cells, BMMSCs and ADSCs respectively.

Key words: Poly (Nisopropylacrylamide) (PNIPAAm), Bone marrow mesenchymal stem cells (BMMSCs), Adiposederived stem cells (ADSCs), Temperaturesensitive materials, Nonenzymatic cell recovery

杨磊,王战勇,范晓光. 适宜不同种类细胞的温敏性共聚物膜的筛选[J]. 辽宁石油化工大学学报, 2014, 34(3): 14-18.

Yang Lei，Wang Zhanyong，Fan Xiaoguang. Selection of TemperatureResponsive Copolymer Film for Different Cells[J]. Journal of Liaoning Petrochemical University, 2014, 34(3): 14-18.

适宜不同种类细胞的温敏性共聚物膜的筛选

Selection of TemperatureResponsive Copolymer Film for Different Cells

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