Polymer microspheres are used extensively in oil field which can improve water flooding efficiency of deep profile control agent. In order to better analyze and adjust the effect, an oilfield reservoir geology and characteristic of exploitation and dynamic performance of polymer microspheres after polymer flooding production were classified analysis. The reservoir adaptability and effective characteristics were also investigated. The adaptability of polymer microspheres flooding was analyzed mainly from geological factors, microspheres matching factors, and engineering factors. The results showed that the main object of tapping was the pore fracture type well group which the moisture content was 30%～50%. The matching relations among microspheres and porous reservoir roar radius and the salinity of formation water were considered, the concentration and amount of slug agents need to evaluate specially. The reservoir screening criteria was established based on the adaptability analysis of low permeability reservoir polymer microspheres. The specific screening index limit of formation water mineralization degree, reservoir permeability, and reservoir thickness were given, it provided great insurance for selective microsphere polymer flooding block.