功能菌群对柴油污染土壤的修复及菌群演替特性

doi:10.12422/j.issn.1006-396X.2023.04.003

石油化工高等学校学报 ›› 2023, Vol. 36 ›› Issue (4): 20-24.DOI: 10.12422/j.issn.1006-396X.2023.04.003

功能菌群对柴油污染土壤的修复及菌群演替特性

赵中汀(), 李爽, 朱春昀, 马会强()

辽宁石油化工大学环境与安全工程学院，辽宁抚顺 113001

收稿日期:2022-04-12 修回日期:2023-02-25 出版日期:2023-08-25 发布日期:2023-09-05
通讯作者: 马会强
作者简介:赵中汀（1997⁃），女，硕士研究生,从事石油烃污染场地修复技术方面的研究；E⁃mail：ting1997@126.com。
基金资助:
辽宁省“兴辽英才计划”项目(XLYC2007007)

Remediation of Diesel Contaminated Soil by Functional Flora and Succession Characteristics

Zhongting Zhao(), Shuang Li, Chunyun Zhu, Huiqiang Ma()

School of Environmental and Safety Engineering，Liaoning Petrochemical University，Fushun Liaoning 113001，China

Received:2022-04-12 Revised:2023-02-25 Published:2023-08-25 Online:2023-09-05
Contact: Huiqiang Ma

摘要/Abstract

摘要：

利用人工模拟柴油污染土壤，研究了功能菌群对柴油污染土壤的降解修复能力，以及菌群多样性和菌群结构组成变化。菌群对土壤中柴油的降解实验结果表明，降解30 d后，菌群对土壤中柴油污染的最终降解率达到74.3 %；在降解时间小于30 d时，土壤中菌群的呼吸强度逐渐增大；当土壤深度改变时，菌群的降解能力随深度的增加而逐渐减弱。高通量测序结果表明，降解30 d后，土壤中微生物多样性和丰富度较降解初始都有所增加，说明菌群可以良好地适应柴油污染环境；降解初始柴油降解菌群主要由变形菌门（Proteobacteria）、硬壁菌门（Frmicutes）以及少量拟杆菌门（Bcteroidetes）组成，菌群经30 d培养后，表层土壤中的主要优势菌群为Proteobacteria，深层土壤中的主要优势菌群为Firmicutes。研究菌群对柴油污染土壤的修复及菌群多样性和菌群结构组成变化，可为修复柴油污染土壤提供技术支持。

关键词: 土壤修复, 柴油降解菌群, 高通量测序, 多样性

Abstract:

Using artificial simulation of diesel contaminated soil, the degradation and remediation ability of functional microbial communities on diesel contaminated soil, as well as changes in microbial diversity and microbial structure composition, were studied. The experimental results of microbial community degradation of diesel in soil showed that after 30 days of degradation, the final degradation rate of microbial community on diesel pollution in soil reached 74.3%.The respiratory intensity of the microbial community in the soil gradually increases within 30 days. When the soil depth changes, the degradation ability of the microbial community gradually weakens with the increase of depth. The results of high throughput sequencing showed that after 30 days of degradation, the microbial diversity and richness in the soil increased compared with the initial degradation, indicating that the microbial community could adapt to the diesel pollution environment well. The initial microbial community for diesel degradation is mainly composed of Proteobacteria, Firmicutes, and a small amount of Bctoidetes. After 30 days of cultivation, the main dominant bacteria in the surface soil are Proteobacteria, while the main dominant bacteria in the deep soil are Firmicutes. Studying the remediation of diesel?contaminated soil by microbial communities and changes in microbial structure and diversity, technical support can be provided for the remediation of diesel?contaminated soil.

Key words: Soil remediation, Diesel degrading bacteria, High throughput sequencing, Diversity

中图分类号:

TQ09

赵中汀, 李爽, 朱春昀, 马会强. 功能菌群对柴油污染土壤的修复及菌群演替特性[J]. 石油化工高等学校学报, 2023, 36(4): 20-24.

Zhongting Zhao, Shuang Li, Chunyun Zhu, Huiqiang Ma. Remediation of Diesel Contaminated Soil by Functional Flora and Succession Characteristics[J]. Journal of Petrochemical Universities, 2023, 36(4): 20-24.

图/表 5

图1 投加菌群前后土壤中柴油降解率的变化

Fig.1 Changes of diesel degradation rate in soil before and after adding flora

图2 投加菌群前后土壤中CO2质量分数的变化

Fig.2 Changes in the cumulative release of CO2 in soil before and after adding flora

图3 柴油在不同土壤深度的质量分数

Fig.3 Mass fraction of diesel at different depths in soil

表1 Alpha多样性分析

Table 1 Alpha diversity analysis

样本

Shannon

指数

Chao指数

Coverage

指数

图4 相对丰度3D柱状图

Fig.4 3D histogram of relative abundance

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功能菌群对柴油污染土壤的修复及菌群演替特性

Remediation of Diesel Contaminated Soil by Functional Flora and Succession Characteristics

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