Journal of Petrochemical Universities
  Journals
  Publication Years
  Keywords
Search within results Open Search
Please wait a minute...
For Selected: Toggle Thumbnails
Application of Thermophiles in Microbial Enhanced Oil Recovery
SUN Shan-shan, ZHANG Zhong-zhi,LU Mang, et al
Abstract1035)      PDF (488KB)(461)      
The application of three thermophilic bacteria (named GW1, GW2, GW5, respectively) in microbial enhanced oil recovery (MEOR) was studied. The results show that the optimal temperature of three bacterium is 55 to 65 ℃, the optimum pH value is between 7 and 8, and the optimal salinity is 5 000-20 000 mg/L. GW1 is sensitive to sulphonate cleanup additive and frothing agent, frothing agent for GW2 is disincentive, and GW5 is susceptible to sulphonate cleanup additive. Thermophilic bacterium degraded the heavy components to light ones and increased the mobility of crude oil. GW1, GW2 and GW5 are identified as [WTBX]Geobacillus.
 
2009, 22 (4): 41-44. DOI: 10.3696/j.issn.1006-396X.2009.04.011
Application of Green Fluorescent Protein as Gene Marker for Microbial Enhanced Oil Recovery
ZHANG Zhong-zhi, SUN Shan-shan, WANG Yu-xiao,et al
Abstract2294)      PDF (345KB)(499)      
In order to obtain more analyses and estimate correctly and accurately to improve Microbial Enhanced Oil Recovery (MEOR), goal strains for MEOR would be marked and tracked to indicate that how and where they moved and reproduced in oil reservoir and show their amount and rate in oil-field microorganism. Recombinant plasmid of pET-30a(+)-EGFP which has the gene of green fluorescent protein (GFP) was constructed and transferred into JD successfully . Recombinant plasmid can be stably maintained in the culture media including 50 μg/mL kanamycin (Kan), which indicated recombinant plasmid stability under choice pressure, while without kanamycin it can be lost easily. JD(gfp) can normally reproduce and metabolize together with wild strain for MEOR isolated from oil reservoir while no transfer of recombinant plasmid.
2009, 22 (4): 34-37. DOI: 10.3696/j.issn.1006-396X.2009.04.009
 
Construction of a Genetic Engineering Strain That Can Produce Extracellular Polyssacharidesin Higher Temperature for MEOR
YI Wen-jing, ZHANG Zhong-zhi*,SUN Shan-shan, WEI Xiao-fang, LUO Yi-jing
Abstract286)      PDF (1051KB)(317)      
 
A strain screened from the waste water of an oilfield can produce insoluble extracellular polysaccharide used in selective plugging which is instrumental in microbially enhanced oil recovery. However this strain is sensitive to high temperature and produces little extracellular polyssacharides above 40 ℃. Therefore pET-ecoRRF, pET-tteRRF and corresponding control plasmids were transformed into the strain. The new strain can grow in 45 ℃, and western-blot indicates that the RRF genes have been expressed noticeably in the transgene strains. It has been showed that the transgene strains are able to bear higher temperature, and the sustainable temperature is higher five to eight degrees than the original strain. Because Enterobacter sp. is related to Escherichia coli, the expression of RRF is better than the gene of Thermus thermophilus in the strain of JD. At last it is successful to get a genetic engineering microbe which can endure the temperature as high as 45 ℃ and produce extracellular polysaccharides.
2007, 20 (4): 25-27.