石油化工高等学校学报

石油化工高等学校学报 ›› 2010, Vol. 23 ›› Issue (4): 1-4.DOI: 10.3696/j.issn.1006-396X.2010.04.001

• 石油化工 •    下一篇

3株低温烷烃降解菌的摄取模式

马会强1,张洪林1,李爽1,李长波1,刘娜2   

  1. 1.辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001;2.吉林大学环境与资源学院,吉林长春130026
  • 收稿日期:2010-09-07 修回日期:2010-10-10 出版日期:2010-12-25 发布日期:2010-12-25
  • 作者简介:马会强(1982 -), 男, 黑龙江萝北县, 讲师, 博士
  • 基金资助:
    国家“ 863” 计划项目(2007AA06Z343)

Uptake Modes on Three Types of Low Temperature Alkanes-Degrading Microbes

  1. 1. School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun Liaoning 113001, P. R. China;
    2. College of Environment and Resources, Jilin University, Changchun Jilin 130026, P. R. China
  • Received:2010-09-07 Revised:2010-10-10 Published:2010-12-25 Online:2010-12-25

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摘要： 采用碳源利用性、细胞表面亲油性和发酵液乳化性方法研究了3 株低温烷烃降解菌:A(耶氏酵母菌)、H(红球菌)及LD2(不动杆菌)对石油烃的摄取模式。结果表明, A、H 菌利用亲水性碳源能力较强, 稳定期发酵液OD600 值分别为1.85 ～ 2.12 、1 .41 ～ 1.54 , 同时菌株能较好的利用疏水性碳源生长, 发酵液OD600 值分别为0 .68 ～ 0.80、0.63 ～ 0 .68 ;菌株细胞表面亲油性在对数生长期最高, M ATH 值分别为0 .69 、0.61 ;菌株均产生表面活性物质, 使油层乳化呈微滴悬浮于水相, 乳化层与总高度比值最大分别为0 .94 、0 .96;确定A 、H 菌摄取石油烃时属于细胞与石油烃微滴之间的相互作用模型。LD2 菌利用亲水性碳源能力较差, 稳定期发酵液OD600 值仅为0 .48 ～ 0.58, 但能较好的利用疏水性碳源生长, 发酵液OD600 值为0.78 ～ 0 .82;菌株细胞表面亲油性在整个生长周期均较高, MATH 值为0 .72 ～ 0.85 ;油层无乳化;确定LD2 菌在摄取石油烃时属于细胞与石油烃大颗粒之间的直接接触模型。

关键词: 低温 , 烷烃降解菌 , 摄取模式 , 细胞表面亲油性

Abstract: Carbon energy utilization, cell surface hydrophobicity and broth emulsion capability methods were adopted to study uptake modes on three types of low temperature alkanes-degrading microbes, A(Yarrowia sp.), H(Rhodococcus sp.), LD2(Acinetobacter sp.) to assimilate petroleum hydrocarbon. The results show that microbe A and H exhibited good capability to utilize hydrophilic carbon energy, broth OD600 values are 1.85～2.12 and 1.41～1.54 respectively at stale period, and microbes also utilized hydrophobic carbon energy well, broth OD600 values are 0.68～0.80 and 0.63～0.68. Microbe cell surface hydrophobicity reached maximum values at logarithmic growth phase, MATH values are 0.69 and 0.61. Microbes can produce surfactant to make oil layer emulsion and presented large amount of microdroplets suspended in water phase, the maximum height ratio of emulsion layer to total layers are 0.94 and 0.96, so uptake mode on microbe A and H belong to interaction between cell and petroleum microdroplets. Microbe LD2 has poor capability to utilize hydrophilic carbon energy, broth OD600 values are only 0.48～0.58 at stable period, but LD2 [KG*4]utilized [KG*4]hydrophobic [KG*4]carbon [KG*4]energy [KG*4]well, [KG*4]broth OD600 values are 0.78～0.82. Microbe had a higher cell surface hydrophobicity at all growth periods, MATH values are 0.72~0.85, oil layer demonstrated none emulsion, therefore, uptake mode on microbe LD2 belongs to direct contact between cell and petroleum large particles.

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引用本文

马会强,张洪林,李爽,李长波,刘娜. 3株低温烷烃降解菌的摄取模式[J]. 石油化工高等学校学报, 2010, 23(4): 1-4.

MA Hui-qiang,ZHANG Hong-, LI Shuang. Uptake Modes on Three Types of Low Temperature Alkanes-Degrading Microbes[J]. Journal of Petrochemical Universities, 2010, 23(4): 1-4.

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